Fully-automated Immunogold Labeling of Resin Embedded Specimens and On-grid Deposition of Gold Fiducial Particles
نویسندگان
چکیده
Immunogold labeling (IGL) was introduced in the 1970s as a method for localizing the protein constituents of cells in electron micrographs, through the attachment of antibody-bound colloidal gold particles to antigens on the surfaces of plastic-embedded EM samples. Here we demonstrate use of the Microscopy Innovations mPrepTM System ASP-1000 Automated Specimen Processor to perform unattended, fully-automated IGL grid preparation using an AurionTM suggested protocol and immuno reagents.
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